Human IL-15 Fluorescein MAb (Clone 34559) (100 TESTS)【科瑞奥博】

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Human IL-15 Fluorescein MAb (Clone 34559) (100 TESTS)

货号： IC2471F 基本售价： 3390.7 元规格： 100 Tests

产品信息

概述
货号	IC2471F
别名	IL-15MGC9721; interleukin 15; interleukin-15
全称	Interleukin 15
反应种属	Human
应用	Intracellular Staining by Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2, recombinant mouse IL-15, or rhIL-21 is observed.
使用方法	Intracellular Staining by Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Phenotypic and Functional Characterization of NK Cells in Human Immune Response against the Dimorphic Fungus Paracoccidioides brasiliensis. Authors: Longhi LN, da Silva RM, Fornazim MC, Spago MC, de Oliveira RT, Nowill AE, Blotta MH, Mamoni RL J. Immunol., 2012;189(2):935-45. Species: Human Sample Type: Whole Cells Application: Flow Herpes simplex virus-1 up-regulates IL-15 gene expression in monocytic cells through the activation of protein tyrosine kinase and PKC zeta/lambda signaling pathways. Authors: Ahmad R, Ennaciri J, Cordeiro P, El Bassam S, Menezes J J. Mol. Biol., 2006;367(1):25-35. Species: Human Sample Type: Whole Cells Application: Flow Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs. Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11. Species: Human Sample Type: Whole Cells Application: Flow IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E J. Immunol., 2004;173(2):1463-76. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from ascites
免疫原	E. coli-derived recombinant human IL-15 Asn49-Ser162 Accession # P40933
生物活性	Human
标记	Fluorescein
背景	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11‑13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Grabstein, K. et al. (1994) Science 264:965. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. Dubois, S. et al. (2002) Immunity 17:537. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

参考图片

Detection of IL‑15 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with LPS were stained with Mouse Anti-Human IL‑15 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2471F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.