Human Integrin alpha 3/CD49c Fluorescein MAb (Clone IA3) (100 TESTS)
货号：
FAB1345F 基本售价：
3242.0 元 规格：
100 Tests
产品信息
概述| 货号 | FAB1345F |
| 别名 | antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells)
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| 目标/特异性 | Detects human Integrin alpha 3/CD49c. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3675 (Human); 16400 (Mouse) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering.
Authors: Pytlik R, Stehlik D, Soukup T, Kalbacova M, Rypacek F, Trc T, Mulinkova K, Michnova P, Kideryova L, Zivny J, Klener P, Vesela R, Trneny M, Klener P
Biomaterials, 2009;30(20):3415-27.
Species: Human
Sample Type: Whole Cells
Application: Flow |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human milk epithelial cell line |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1 - 3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130 - 150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (8). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
- Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
- Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
- Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
- de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
- Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
- Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
- Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
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参考图片Detection of Integrin alpha 3/CD49c in HT1080 Human Cell Line by Flow Cytometry. HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human Integrin alpha 3/CD49c Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1345F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
