| 货号 | FAB6321P |
| 别名 | B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform; CD40; CD40L receptor; CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects human CD40/TNFRSF5 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhEDAR, rhFas, rhGITR, rhHVEM, rhLTR beta, rhNGF R, rhOPG, rhRANK, rhTAJ, rhTNF RI, rhTNF RII, or recombinant mouse CD40. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 958 (Human); 21939 (Mouse); 171369 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Oxidation of ovarian epithelial cancer cells by hypochlorous acid enhances immunogenicity and stimulates T cells that recognize autologous primary tumor. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD40/TNFRSF5 Glu21-Arg193 Accession # P25942 |
| 生物活性 | Human |
| 标记 | Phycoerythrin |
| 背景 | CD40, also known as TNFRSF5, is a 45-50 kD type I transmembraneglycoprotein that belongs to the TNF receptor superfamily. Mature human CD40 is257 amino acids (aa) in length, and consists of a 173 aa extracellular domain(ECD), a 22 aa transmembrane segment and a 62 aa cytoplasmic region. The ECDcontains four cysteine-rich repeats, the second of which is considered crucialto ligand binding. Over aa 21-193, humanCD40 shares 58% aa sequence identity with mouse CD40. Although not ubiquitously expressed, cells knownto constitutively or inducibly express CD40 are large in number and includebasal layer keratinocytes, adipocytes, CD8+ T cells, fibrocytes (circulatingstem cells), monocytes, eosinophils, dendritic cells, pancreatic ductepithelium, astrocytes, smooth muscle cells, fibroblasts, vascular endothelialcells and B cells. There is at least onealternative splice form that shows a 38 aa substitution for aa 166-277,generating a soluble molecule. There aretwo "ligands" for CD40, the first being CD154/CD40L, and the second Borrelia, aspirochete that causes Lyme disease. CD40 ligation by CD154 has many outcomes, some of which arecell-specific. Ligation of CD40 on Bcells (with IL-4) induces IgE production, while bone marrow stromal cellactivation via CD40 promotes OPG secretion with concomitant boneformation. In addition, stimulation ofCD40 on keratinocytes results in IL-8 and RANTES expression with subsequentleukocyte infiltration, while adipocyte activation via CD40 promotes local M1polarization. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD40/TNFRSF5 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD40/TNFRSF5 PE‑conjugated Monoclonal Antibody (Catalog # FAB6321P) and Mouse Anti-Human CD19 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB4867F). Quadrant markers were set based on control antibody staining (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins. |
