Human E-Selectin/P-Selectin Fluorescein MAb (Clone BBIG-E6) (100 TESTS)
货号：
FAB6169F 基本售价：
2676.9 元 规格：
100 Tests
产品信息
概述| 货号 | FAB6169F |
| 别名 | E-Selectin (CD62E)/P-Selectin (CD62P); Eselectin; PSelectin |
| 反应种属 | Human |
| 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Authors: Lauridsen H, Pober J, Gonzalez A
FASEB J, 2014;28(3):1166-80.
Species: Human
Sample Type: Whole Cells
Application: ICC |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Activated HUVEC human umbilical vein endothelial cells |
| 生物活性 | Human |
| 标记 | Fluorescein |
| 背景 | E-Selectin (CD62E) and P-Selectin (CD62P) are members of the Selectin/LECAM family of molecules. E- and P-Selectin are type I transmembrane glycoproteins that contain an N-terminal calcium-dependent C-type lectin domain, one EGF-like repeat, multiple sushi domains, and a transmembrane segment followed by a short cytoplasmic tail. Typically, the ligands for E-and P-Selectin are carbohydrates that contain a sialyl-Lewis X (sLeX) motif that appears on select O- and N-linked glycans and glycosphingolipids. Human E-Selectin is 95-115 kDa is size and expressed by vascular endothelium. It has a number of ligands, not all of which are shared between the human and rodent systems. For example human E-Selectin binds L-Selectin, CD44/HCELL and a poorly characterized pronase-insensitive ganglioside, none of which are recognized by mouse E-Selectin. By contrast, mouse E-Selectin binds E-Selectin Ligand whereas human E-Selectin does not. Other ligands identified for human E-Selectin include PSGL-1, CD43, Mac-1, CD66, Endoglycan and Podocalyxin-like protein. Human P-Selectin is 130-140 kDa in size, and expressed platelets and vascular endothelium. It primarily binds sLeX on O-glycans, including PSGL-1 and endoglycan. Functionally, Selectins induce leukocyte rolling on endothelial cell surfaces, an effect that activates neighboring alpha 2 beta 2/LFA-1 and slows leukocyte migration. Although the extracellular domains of human E- and P-Selectin show only 36% amino acid (aa) sequence identity, over the first 180 aa (out of >500 aa), E- and P- Selectin demonstrate 62% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Endothelial Cell Differentiation Markers
- Endothelial Cell Markers
- Endothelial Cell Markers and Adhesion Molecules in Angiogenesis
- Endothelial Progenitor and Endothelial Cell Markers
- ICC / IHC Images: E-Selectin (CD62E)/P-Selectin (CD62P)
- Lectins
- Mini-review: Adhesion Molecules II
- Mini-review: Multiple Sclerosis: Immune Cell Access to the Central Nervous System
- Regulatory T Cells (Tregs)
- Selectins
|
参考图片Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Umbilical Vein Endothelial Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells stained with TNF-alpha was stained with Mouse Anti-Human E‑Selectin/P‑Selectin (CD62E/P) Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB6169F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
