| 货号 | FAB2546B-025 |
| 别名 | Fc gamma RIII; FcgRIII | 全称 | Fc gamma Receptor III |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 14131 (Mouse); 102140945 (Cynomolgus Monkey) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16) Thr20-Gln208 Accession # O75015 |
| 生物活性 | Human |
| 标记 | Biotin |
| 背景 | CD16 (also known as FcgRIII)is a CD designation that, in human, encompasses two related, but distinct Igsuperfamily genes; CD16a and CD16b. Mature human CD16a is a 45-75 kDa type I transmembrane glycoprotein thatis 238 amino acids (aa) in length. Itcontains a 192 aa extracellular region that possesses two Ig-like domains plusglycosylation that is critical to its function, and ends with a short 25 aacytoplasmic domain. On the cell surface,CD16a complexes with FceRI and CD3 zeta. CD16a shows a restricted expression pattern, being found on immaturethymocytes, placental trophoblast cells, CD56dim andcytokine-activated CD56+ NK cells, slan (sulfated NAcLac)-expressing dendritic cells, and CX3CR1+CD14+/- "patrolling" monocytes. Mature CD16b, by contrast, is slightly smaller that CD16a, but stillshows the same 50-70 kSDa electrophoretic pattern in SDS-PAGE. It is a GPI-linked glycoprotein that is 184aa in length. On the cell surface, CD16bis monomeric. The standard allele (=NA2) is encoded by the SwissProt Accession # used for this product (seeabove). The two variant alleles are NA1,which shows four aa substitutions, and SH, which contains only one aasubstitution relative to the NA2 sequence. CD16b expression appears to be limitedto neutrophils. CD16b is known to besolubilized, and the soluble form is believed to arise from the action ofADAM-17 rather than a phospholipase. Over aa 20-208, human CD16a and CD16b share 97% aa sequenceidentity. Over the same aa range, humanCD16b and mouse CD16 share only 47% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A) and either (A) Mouse Anti-Human Fc gamma RIII (CD16) Biotinylated Monoclonal Antibody (Catalog # FAB2546B) or (B) Mouse IgG2A Biotinylated Isotype Control (Catalog # IC003B) followed by Streptavidin-Phycoerythrin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins. |
