货号 | FAB2546C-025 |
别名 | Fc gamma RIII (CD16); FcgRIII | 全称 | Fc gamma Receptor III |
反应种属 | Human |
应用 | Flow Cytometry(10 µL/106cells) |
目标/特异性 | Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed. |
使用方法 | Flow Cytometry: 10 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 14131 (Mouse); 102140945 (Cynomolgus Monkey) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16) Thr20-Gln208 Accession # O75015 |
生物活性 | Human |
标记 | PerCP |
背景 | Fc gamma RIIIA and B are low affinity receptors for polyvalentimmune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptorexpressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIBis a non-activating GPI-linked decoy receptor expressed on neutrophilsand eosinophils. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Fc gamma RIII (CD16) in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) PerCP‑conjugated Monoclonal Antibody (Catalog # FAB2546C) and Mouse Anti-Human CD14 APC‑conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins. |