Human CD25/IL-2 R alpha Phycoerythrin MAb (Clone 24212) (25 TESTS)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human CD25/IL-2 R alpha Phycoerythrin MAb (Clone 24212) (25 TESTS)

货号： FAB1020P-025 基本售价： 1259.1 元规格： 25 Tests

产品信息

概述
货号	FAB1020P-025
别名	CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR
全称	Interleukin 2 Receptor alpha
反应种属	Human
应用	Flow Cytometry(10 µL/10⁶cells)
目标/特异性	Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R gamma or rhIL‑15 R alpha is observed.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration. Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F J Immunol, 2014;192(1):123-35. Species: Human Sample Type: whole cells Application: Flow Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. Authors: Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A 2011;6(5):e19988. Species: Human Sample Type: Whole Cells Application: Flow VEGFR2 is selectively expressed by FOXP3high CD4+ Treg. Authors: Suzuki H, Onishi H, Wada J, Yamasaki A, Tanaka H, Nakano K, Morisaki T, Katano M Eur. J. Immunol., 2010;40(1):197-203. Species: Human Sample Type: Whole Cells Application: Flow Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Authors: Ni Z, Walcheck B Clin. Immunol., 2009;133(2):257-64. Species: Human Sample Type: Whole Cells Application: Flow Histone deacetylase inhibitors suppress natural killer cell cytolytic activity. Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J FEBS Lett., 2007;581(7):1317-22. Species: Human Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha Glu22-Cys213 Accession # P01589
生物活性	Human
标记	Phycoerythrin
背景	Human CD25, also known as IL-2 receptor alpha (IL-2 R alpha ) and asTac antigen, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2. The IL-2 R alpha cDNA encodes a 272 amino acid residue precursor Type I membrane protein with a 21 residue signal peptide, a 219 residue extracellular region, a 19 residue transmembrane region and a 13 residue cytoplasmic domain. IL-2 R alpha lacks structural features characteristic of members of the cytokine receptor superfamily. By itself, IL-2 R alpha binds IL-2 with low affinity. However, when IL-2 R alpha is associated with the IL-2 receptor beta and gamma chains, a high affinity heterotrimeric receptor complex that transduces IL-2 signals is formed. Soluble forms of many cytokine receptors have been reported, and a soluble form of IL-2 R alpha (IL-2 sR alpha ) appears in serum, concomitant with its increased expression on cells. The function of the soluble IL-2 R alpha is unclear. Increased levels of IL-2 sR alpha in biological fluids reportedly correlate with increased T and B cell activation and immune system activation. Increased serum concentration of IL-2 sR alpha has been observed in patients with a variety of inflammatory conditions and in the course of some leukemias and lymphomas.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Lymphoblastic Leukemia Acute Myeloid Leukemia B Cell Activation B Cell Markers Cancer Biomarkers Common gamma Chain Receptor Family Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Tfh Cell Differentiation and Function Group 1 ILCs Group 2 ILCs Human CD Marker Products ICC / IHC Images: CD25/IL-2 R alpha Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types Isolation, Culture, and Identification of Natural Killer Cells LTi Cells Lymphoma NCR+ILC3 NCR-ILC3 Neuroinflammation Preeclampsia Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs) T Cell Cytokine Signaling

参考图片

Detection of Human CD25/IL-2 R alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 20 ng/mL Recombinant Human IL‑2 (Catalog # 202-IL) and 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog #
240-B) for 2 days were stained with Mouse Anti-Human CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB3791A) and either (A) Mouse Anti-Human CD25/IL-2 R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1020P) or (B) Mouse IgG_2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.