| 货号 | FAB3791T-025 |
| 别名 | CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects human CD4 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse CD4. |
| 使用方法 | Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Recombinant human CD4 Extracellular domain Accession # P01730 |
| 生物活性 | Human |
| 标记 | Alexa Fluor 594 |
| 背景 | CD4 is a 54-60 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It is expressed predominantly on hematopoietic cells such as T cells, monocytes and Langerhans cells. T cells known to be positive for CD4 include Th1, Th2, Th17, Th22, Th9, Tfh and Treg subsets, plus select thymocytes. CD4 exists as either an oxidized monomer (3 internal disulfide bonds), a reduced monomer (2 internal disulfide bonds) and a disulfide-linked dimer. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. In particular, CD4 in its dimeric form binds to the MHC complex at non-polymorphic sites. Monomeric CD4 has also been shown to be a coreceptor for HIV entry and specifically to bind gp120, the external envelope glycoprotein of HIV. Over amino acids (aa) 26-396 (the extracellular domain), human and mouse CD4 share 58% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD4 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB3791T) or (B) Mouse IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC003T). View our protocol for Staining Membrane-associated Proteins. |
