货号 | FAB3791N-025 |
别名 | CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 |
反应种属 | Human |
应用 | Flow Cytometry |
目标/特异性 | Detects human CD4 in direct ELISAs. |
使用方法 | Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | Recombinant Human CD4. Extracellular Domain Accession # P01730 |
生物活性 | Human |
标记 | Alexa Fluor 700 |
背景 | CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of CD4 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD4 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB3791N) or (B) Mouse IgG2A Alexa Fluor 700 Isotype Control (Catalog # IC003N). View our protocol for Staining Membrane-associated Proteins. |