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货号： bs-0772R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-0772R
- 英文名称
- phospho-FSCN1 (Ser39)
- 中文名称
- 磷酸化纤维束蛋白同源物1抗体
- 别 名
- Fascin (phospho Ser39); Fascin (phospho S39); 55 kDa actin bundling protein; Actin bundling protein; FAN 1; FAN1; Fascin 1; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin1; FLJ38511; FSCN 1; FSCN1; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like protein; SNL; Strongylocentrotus purpuratus.
- Specific References (2) | bs-0772R has been referenced in 2 publications.[IF=3.73] Majchrzak K, Lo Re D, Gajewska M, Bulkowska M, Homa A, et al. (2013) Migrastatin Analogues Inhibit Canine Mammary Cacer Cell Migrationand Invasion. PLoS ONE 8(10): e76789 Dog.PubMed:24116159[IF=3.31] Król, Magdalena, et al. "Macrophages Mediate a Switch between Canonical and Non-Canonical Wnt Pathways in Canine Mammary Tumors." PloS one 9.1 (2014): e83995. Dog.PubMed:24404146
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 细胞生物 信号转导 结合蛋白
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 55kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human FSCN1 around the phosphorylation site of Ser39:AS(p-S)LK
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However, Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.
Function:
Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration.
Subcellular Location:
Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection >invadopodium.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin.
Similarity:
Belongs to the fascin family.
SWISS:
Q16658
Gene ID:
6624
Database links:Entrez Gene: 6624 Human
Entrez Gene: 14086 Mouse
Entrez Gene: 683788 Rat
Omim: 602689 Human
SwissProt: Q16658 Human
SwissProt: Q61553 Mouse
SwissProt: P85845 Rat
Unigene: 118400 Human
Unigene: 289707 Mouse
Unigene: 199526 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
肌动蛋白集束蛋白/圆线虫紫癜抗体。
FSCN1为细胞骨架肌动蛋白的一种，该蛋白有蛋白结合,桥连、肌动蛋白丝结合的功能。 主要参与细胞增殖、肌动蛋白细胞骨架组织和生物发生、肌动蛋白丝束形成。<
- 产品图片
Sample:
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-FSCN1(Ser39) (bs-0772R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FSCN1(Ser39)) Polyclonal Antibody, Unconjugated (bs-0772R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: musle of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-FSCN1(Ser39) Polyclonal Antibody, Unconjugated(bs-0772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 3μl in 100 μL1X PBS containing 0.5% BSA(green).