Hu Caspase-6 Pure B93-4 100ug
货号：
556581 基本售价：
5092.0 元 规格：
100ug
产品信息
概述| 货号 | 556581 |
| 描述 | The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. ICE (interleukin-1ß converting enzyme) was the first member of this family to be discovered following a search for human proteins with homology to ced-3, a cell death gene identified in C. elegans. "Caspase" has been adopted as a root name for all family members based on the properties of these enzymes. The "c" reflects a cysteine protease mechanism and "aspase" refers to their ability to cleave after aspartic acid residues. Caspases are synthesized as inactive proenzymes that are processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms consist of large (17-22 kDa) and small (10-12 kDa) subunits which 34kDa associate to form an active enzyme. Caspase-6 exists as two isoforms, designated Mch2 α (caspase-6) and Mch2ß, a proteolytically inactive isoform which lacks half of the large subunit.3 Caspase-6 is most closely related to caspase-3, and can be proteolytically cleaved/activated by caspase-3 into large (p18) and small (p11) subunits. Similarly, caspase-3 has been shown to be activated by caspase-6, suggesting that these proteases may generate an amplification cycle during apoptosis. Other proteolytical targets of Western blot analysis of caspase-6. In lane 1, active caspase-6 include lamin A and a cytosolic nuclease which translocates to the Daudi B cell lysate was probed with anti-nucleus to promote DNA fragmentation. Caspase-6 has also been shown to cleave Fak (focal adhesion kinase), a non-receptor protein tyrosine kinase that transduces cell Clone B93-4 identifies full length caspase-6 at survival and proliferation signals from contact sites between the cell surface and extracellular matrix. In this instance, caspase-6 cleavage inhibits the anti-apoptotic function of Fak. Therefore, the protease activity of caspase-6 plays both activating and inhibitory roles in apoptotic pathways. Caspase-6 is observed at ~34 kDa in SDS-PAGE. The B93-4 clone recognizes human caspase-6. The antibody detects both full length caspase-6 (34 kDa), as well as the p11 subunit of the active enzyme. A synthetic peptide sequence corresponding to amino acids 271-285 (KKQVPCFASMLTKK) of human caspase-6 was used as immunogen. |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human Caspase-6 aa. 271-285 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
