Hu Caspase-8 Pure B9-2 100ug
货号：
556466 基本售价：
5092.0 元 规格：
100ug
产品信息
概述| 货号 | 556466 |
| 描述 | Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transducer, FADD/MORT-1, as well as to other caspase (ICE/Ced-3) cysteine proteases. The N-terminal region of Caspase-8 contains an amino acid sequence, termed the death domain, that facilitates Caspase-8-FADD direct interaction. FADD therefore acts as an adapter molecule, allowing Caspase-8 to become recruited to the cytoplasmic region of Fas following receptor activation. Viral proteins (v-FLIPS) which inhibit recruitment and activation of Caspase-8 have been isolated. Caspase-8 is produced as a proenzyme which upon receptor aggregation is proteolytically cleaved into 20 kDa and 10 kDa subunits. These subunits form a proteolytically active heterodimer capable of cleaving other Caspase family members, including PARP. Unlike other caspase family members, overexpression of FLICE is sufficient to induce apoptosis in certain cell lines (e.g., MCF-7) and this phenotype is blocked by overexpression of the Caspase-3 protease inhibitor, CrmA. The B9-2 antibody recognizes an ~55 kDa band corresponding to human Caspase-8 (FLICE). A recombinant human Caspase-8 protein fragment corresponding to amino acids 335-469 was used as immunogen. |
| 别名 | FLICE, MACH-1, Mch5 |
| 同种亚型 | IgG2a |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Recombinant Human Caspase-8 aa. 335-469 |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
