Hu p53 Pure DO-7 100ug
货号：
554294 基本售价：
5092.0 元 规格：
100ug
产品信息
概述| 货号 | 554294 |
| 描述 | p53 is a 53 kD nuclear phosphoprotein that acts as a tumor suppressor protein, and is involved in inhibiting cell proliferation when DNA damage occurs. The gene for p53 is the most commonly mutated gene yet identified in human cancers. Missense mutations occur in tumors of the colon, lung, breast, ovary, bladder and several other organs. The mutant p53 is overexpressed in a variety of transformed cells and wildtype p53 forms specific complexes with several viral oncogenes including SV40 large T, E1B from adenovirus, and E6 from human papilloma virus. Wildtype p53 plays a role as a checkpoint protein for DNA damage during the G1/S-phase of the cell cycle. However, it is still unclear, whether point mutated forms of p53 are simple null mutants and/or dominant negatively acting proteins. The DO-7 antibody recognizes human wildtype and mutant p53. It cross-reacts with bovine p53 but does not cross-react with mouse or rat p53. DO-7 recognizes an epitope between amino acids 1-45 of known forms of human p53. Human recombinant p53 protein was used as immunogen. Wildtype 53 proteins have a very short half-life and are usually not detectable with monoclonal antibodies (mAbs) in normal tissues. |
| 同种亚型 | IgG2b |
| 反应种属 | Human (QC Testing) Monkey, Cow (Tested in Development) |
| 来源宿主 | Mouse |
| 使用方法 | FC, WB, IHC(F), IHC(Fr), IP |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Human Recombinant p53 protein |
| 存放说明 | Store undiluted at 4°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
