Hu E47 FITC G127-32 100ug
货号：
552510 基本售价：
4725.0 元 规格：
100ug
产品信息
概述| 货号 | 552510 |
| 描述 | B lymphoid cells go through many intermediate steps before becoming plasma cells which produce and secrete immunoglobulins (Ig). The earliest stage of B-cell differentiation is represented by pro-B lymphocytes that contain both the Ig heavy- and light-chain genes in the transcriptionally inactive germ line configuration. As pro-B lymphocytes differentiate into pre-B cells, Ig heavy-chain genes rearrange and are transcribed and translated. However, the Ig light-chain genes are not transcribed before the pre-B cells differentiate into mature Blymphocytes. The developmental regulation of the Ig gene expression is dependent on various sequences in the Ig enhancer region. One class of such regulatory sequence elements comprises the E-boxes which share the NNCANNTGNN consensus sequence. The E2 boxes are particularly interesting because they are present in muscle and pancreas-specific enhancers. A family of proteins binds to the E2 box. These proteins share a common amino acid sequence motif that is proposed to form two amphipathic helices interrupted by a loop, designated the helix-loop-helix (HLH) motif. The HLH motif mediates homo- as well as heterodimerization with other HLH proteins. Most HLH proteins possess a basic region located at the N terminal of the HLH region which is responsible for DNA binding. Two E2 box binding proteins have been described (E12 and E47) that arise as alternatively spliced form of the E2A gene. E12 and E47 are identical except in the HLH region. These two proteins have been directly implicated in the regulation of B-cell, muscle and pancreas-specific gene expression. Futhermore, studies have shown that relative levels of E47 are important for regulating the lineage of T cells in the thymus. For example, mice deficient in E47 demonstrate a change in the ratio of CD4/CD8 cells as well as a greater percentage of cells that stain for the mature CD4 marker, implicating E47 in selection of T cells. Clone G127-32 recognizes human E47. It cross-reacts with mouse E47. G127-32 does not recognize E12, the alternately spliced product of the E2A gene or E2-2, a closely reltated protein. Recombinant E47 was used as immunogen. |
| 别名 | E47(E2A), Immunoglobulin E2-Box Binding Protein |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) Mouse (Reported) |
| 来源宿主 | Mouse |
| 使用方法 | FC |
性能| 供应商 | BD Pharmingen |
| 免疫原 | Recombinant Human E47 |
| 标记 | FITC |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
