Hu E2F-2 Pure TFE22 100ug
货号：
556525 基本售价：
3614.0 元 规格：
100ug
产品信息
概述| 货号 | 556525 |
| 描述 | The transcription factor E2F was originally characterized as a sequence-specific DNA-binding factor. E2F is now known to be a family of related factors; E2F-1 through E2F6 have been cloned. E2F proteins form heterodimers with the related proteins, DP-1 and -2. These complexes are regulated by their interaction with the retinoblastoma proteins (Rb, p107 and p130), which restrict E2F/DP complexes to the cytoplasm. E2F inhibitors are phosphorylated during the cell cycle. Phosphorylation of inhibitory proteins releases the E2F/DP complex to enter the nucleus, thus providing regulated activation of E2F responsive genes. E2F-DNA binding sites have been found in the promoter regions of genes important for growth regulation, e.g., c-myc, N-myc, cdc2 and cyclin A and within genes which are important for DNA synthesis. Activation of specific gene(s) may depend on the formation of distinct E2F complexes. Cotransfection studies demonstrate specific interactions between E2F and DP proteins, i.e., E2F-1 through -5 bind to DP-1, while E2F-4 binds to DP-2. The E2Fs also display differential binding to pRb family members. E2F gene products can promote cell cycle progression and proliferation. E2F-1, -2, and -3 are transcriptional activators which by themselves can drive cells through S phase, and can override G1 block; E2F-4 and -5 require interaction with DP to do so. E2F-6 is unique in that it is not regulated by pRb family members and instead acts to repress transcription activation by other E2F members. E2F-2 migrates at a molecular weight of 60 kDa by SDS/PAGE. Clone TFE22 recognizes an epitope between amino acids 1 through 85 of human E2F-2. It does not cross-react with other E2F proteins. The specificity of the antibody was verified by immunoprecipitation of in vitro translated E2F-2 and by western blot analysis of cell extracts. |
| 同种亚型 | IgG1 |
| 反应种属 | Human (QC Testing) |
| 来源宿主 | Mouse |
| 使用方法 | WB |
性能| 供应商 | BD Pharmingen |
| 存放说明 | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
|
| 存储溶液 | Aqueous buffered solution containing ≤0.09% sodium azide. |
| 浓度 | 0.5 mg/ml |
