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货号： bs-6211R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-6211R
- 英文名称
- PCMT1
- 中文名称
- 天门冬氨酸甲基转移酶PCMT抗体
- 别 名
- L isoaspartyl protein carboxyl methyltransferase; L isoaspartyl/D aspartyl methyltransferase; PCMT 1; PIMT; Protein beta aspartate methyltransferase; Protein L isoaspartate (D aspartate) O methyltransferase; PIMT_HUMAN; Protein L isoaspartyl/D aspartyl methyltransferase.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 25kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PCMT1:165-227/227
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
PCMT1 (Protein-L-isoaspartate (D-aspartate) O-methyltransferase) plays a role in the repair and/or degradation of damaged proteins by initiating the conversion of abnormal D-aspartyl and L-isoaspartyl residues to the normal L-aspartyl form. It catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous deamidation, isomerization, and racemization of normal L-aspartyl and L-asparaginyl residues.
Function:
Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).
Subunit:
Monomer.
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.
SWISS:
P22061
Gene ID:
5110
Database links:Entrez Gene: 5110 Human
Entrez Gene: 25604 Rat
Omim: 176851 Human
SwissProt: P22061 Human
SwissProt: P23506 Mouse
SwissProt: P22062 Rat
Unigene: 279257 Human
Unigene: 258431 Mouse
Unigene: 86985 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Hippocampus (Mouse) Lysate at 40 ug
HL60(Human) Cell Lysate at 30 ug
Primary: Anti- PCMT1 (bs-6211R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PCMT1) Polyclonal Antibody, Unconjugated (bs-6211R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.