Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation
Authors: A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson
J Leukoc Biol, 2016;0(0):.
Species: Human
Sample Type: Cell Culture Supernates

IL-27 Suppresses Antimicrobial Activity in Human Leprosy.
Authors: Teles R, Kelly-Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, Modlin R
J Invest Dermatol, 2015;135(10):2410-7.
Species: Human
Sample Type: Cell Culture Supernates

Cytokine and chemokine responses to helminth and protozoan parasites and to fungus and mite allergens in neonates, children, adults, and the elderly.
Authors: Lechner C, Komander K, Hegewald J, Huang X, Gantin R, Soboslay P, Agossou A, Banla M, Kohler C
Immun Ageing, 2013;10(1):29.
Species: Human
Sample Type: Cell Culture Supernates

Differential reconstitution of T cell subsets following immunodepleting treatment with alemtuzumab (anti-CD52 monoclonal antibody) in patients with relapsing-remitting multiple sclerosis.
Authors: Zhang X, Tao Y, Chopra M, Ahn M, Marcus K, Choudhary N, Zhu H, Markovic-Plese S
J Immunol, 2013;191(12):5867-74.
Species: Human
Sample Type: Serum

Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery.
Authors: Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y
Mol. Vis., 2011;17(0):1425-30.
Species: Human
Sample Type: Serum

B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
J. Immunol., 2011;186(0):4518.
Species: Human
Sample Type: Cell Culture Supernates

IL-27 mediates the response to IFN-beta therapy in multiple sclerosis patients by inhibiting Th17 cells.
Authors: Sweeney CM, Lonergan R, Basdeo SA, Kinsella K, Dungan LS, Higgins SC, Kelly PJ, Costelloe L, Tubridy N, Mills KH, Fletcher JM
Brain Behav. Immun., 2010;0(0):.
Species: Human
Sample Type: Cell Culture Supernates

Impact of HIV infection, highly active antiretroviral therapy, and hepatitis C coinfection on serum interleukin-27.
Authors: Guzzo C, Hopman WM, Mat NF
AIDS, 2010;24(9):1371-4.
Species: Human
Sample Type: Serum

Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms.
Authors: Correale J, Farez M
J. Immunol., 2009;183(9):5999-6012.
Species: Human
Sample Type: Cell Culture Supernates

Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28 kDa glycoprotein that is related to the p35 chain of IL-12). The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSxWS-like motif that places the IL-27 molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes.

Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four α-helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and pro-inflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development).