| 货号 | AAI29F |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Chicken |
| 来源宿主 | Goat |
| 应用 | F |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by ELISA. Image caption: Antigen-specific serum antibody responses. NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. | |
Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by ELISA. Image caption: Kinetics of NHC-specific antibody levels following in ovo vaccination. ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. | |
Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by western blotting. Image caption: Western blot analysis of chicken sera. Purified flagellins (FlaA, NHC) and whole cell lysates of C. jejuni 81116 (wild type and ΔflaAB) were analyzed by Western blotting. Pooled sera collected from 18 day-old chickens injected with (A) NHC flagellin (40 μg) or (B) solvent were used as probes and detected with anti-chicken IgY antibody. Molecular mass is indicated in kilodaltons (kDa). The arrowhead indicates the reactivity of the sera with the native C. jejuni FlaA flagellin that is absent in the ΔflaAB mutant strain. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. |
