| 货号 | 5184-2504 |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Sheep |
| 来源宿主 | Rabbit |
| 应用 | C, E, WB |
| 供应商 | Bio-Rad Antibodies |
| 溶解方法 | Reconstitute with 1ml of sterile distilled water. For extended storage use 50% glycerol instead of water. |
| 运输条件 | |
| 存放说明 | Prior to reconstitution store at +4oC. After reconstitution store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of sheep IgG in serum following Toxoplasma gondii infection by ELISA. Image caption: IgG response of the sheep in TLA ELISA in function of time. From: Verhelst D, De Craeye S, Entrican G, Dorny P, Cox E. Parasite distribution and associated immune response during the acute phase of Toxoplasma gondii infection in sheep. BMC Vet Res. 2014 Dec 16;10:293. | |
Published customer image: Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of bound Sheep anti NFκB antibody (VPA00015) by western blotting of HEK293 cell lysates. Image caption: NF-κB p65 protein expression levels (a) Representative immunoblot membrane; (b) Combined densitometry data of three individual experiments, indicating fold changes in the protein expression of NF-κB p65, normalized to the total proteins transferred onto the membrane. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01. From: Serban AI, Stanca L, Geicu OI, Dinischiotu A. AGEs-Induced IL-6 Synthesis Precedes RAGE Up-Regulation in HEK 293 Cells: An Alternative Inflammatory Mechanism? Int J Mol Sci. 2015 Aug 25;16(9):20100-17. | |
Published customer image: Horseradish peroxidase conjugated Rabbit anti Sheep IgG antibody (5184-2504) used for the detection of bound Sheep anti NFκB antibody (VPA00015) in normal human skin derived fibroblasts by western blotting. Image caption: AGEs induced signaling in CCD 1070SK fibroblasts. p-ERK1/2 and NF-κB p65 protein expression levels (a) Representative immunoblot membranes; (b) Combined densitometry data indicating fold changes in the protein expression of p-ERK1/2 (b) and NF-κB p65 (c). Immunoreactive bands were normalized to the total proteins transferred onto the membrane in the corresponding lane. Data are relative to BSA and nonimmune IgG treated cells. BSA: bovine serum albumin; AGEs: advanced glycation end products; * p < 0.05; ** p < 0.01; *** p < 0.001. From: Serban AI, Stanca L, Geicu OI, Munteanu MC, Dinischiotu A (2016) RAGE and TGF-β1 Cross-Talk Regulate Extracellular Matrix Turnover and Cytokine Synthesis in AGEs Exposed Fibroblast Cells. PLoS ONE 11(3): e0152376. |
