| 货号 | VC005-250 |
| 别名 | IgG; immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects heavy ( gamma ) chains on rat IgG and light chains on all rat immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin rat serum proteins or IgG from human or mouse, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC. |
| 使用方法 | Immunohistochemistry: Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Rat IgG |
| 生物活性 | Rat |
| 标记 | HRP Polymer |
| 背景 | VisUCyte™ HRP Polymer is abiotin-free detection reagent which overcomes problems of avidin-biotindetection chemistry, such as endogenous biotin staining and the need to doadditional quenching of endogenous biotin and avidin present insome tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cellsand tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies maybe 2-3 times lower compared to the conventional avidin-biotin-HRP protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of HSP27 in Human Ovary. HSP27 was detected in paraffin-embedded sections human ovary using Rat Anti-Human HSP27 Monoclonal Antibody (Catalog # MAB15801) with Anti-Rat IgG VisUCyte HRP Polymer Antibody (Catalog # VC005). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for the VisUCyte HRP Polymer Detection Reagent. |
