| 货号 | VC006-125 |
| 别名 | IgG; immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 应用 | Immunohistochemistry |
| 目标/特异性 | Detects heavy ( gamma ) chains on sheep IgG and light chains on all sheep immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin sheep serum proteins or IgG from human, mouse or rabbit, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC. |
| 使用方法 | Immunohistochemistry: Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives. |
| 来源 | Polyclonal Donkey IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Sheep IgG |
| 生物活性 | Sheep |
| 标记 | HRP Polymer |
| 背景 | VisUCyte™ HRP Polymer is abiotin-free detection reagent which overcomes problems of avidin-biotindetection chemistry, such as endogenous biotin staining and the need to doadditional quenching of endogenous biotin and avidin present insome tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cellsand tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies maybe 2-3 times lower compared to the conventional avidin-biotin-HRP protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
Detection of Progesterone R/NR3C3 in Human Breast Cancer Tissue. Progesterone R/NR3C3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human Progesterone R/NR3C3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5415) with Anti-Sheep IgG VisUCyte HRP Polymer Antibody (Catalog # VC006). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). View our protocol for the VisUCyte HRP Polymer Detection Reagent. |
