| 货号 | 5597S |
| 来源宿主 | Rabbit |
| 应用 | W/E-P |
| 目标/特异性 | Anti-Biotin (D5A7) Rabbit mAb recognizes biotin attached to proteins, peptides, oligonucleotides, or solid matrices. |
| 使用方法 | E-P () |
| 供应商 | CST |
| 标记 | Biotin |
| 背景 | Biotin is a water-soluble B complex vitamin (vitamin H or B7) that serves as a coenzyme in various metabolic functions. It transfers carbon dioxide for five carboxylase enzymes and is involved in the TCA cycle and gluconeogenesis. In addition, all cells require biotin for cell proliferation, production of fatty acids, and metabolism of fats and amino acids (1). Biotin recycling and attachment to histone proteins is catalyzed by an enzyme named biotinidase. Histone biotinylation is involved in regulating telomere attrition and cellular senescence. Biotinidase deficiency is a rare genetic disorder that can cause neurological and cutaneous symptoms if left untreated (2). Biotin has a high affinity for its natural ligand, avidin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the avidin-biotin complexes (3). This remarkable affinity has been exploited for numerous immunoassays including ELISA, Flow Cytometry, Immunofluorescence, In Situ Hybridization, Affinity Chromatography, and Immunohistochemistry. Anti-Biotin (D5A7) Rabbit mAb can be used as an alternative to avidins in order to minimize background and maximize signal intensity. |
| 存放说明 | -20C |
| 参考文献 | 1 . Zempleni, J. et al. (2010) Biofactors 35, 36-46. 2 . Pindolia, K. et al. (2011) Mol Genet Metab 102, 161-9. 3 . Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30. |
ELISA showing absorbance at 450 nm of biotinylated and non-biotinylated peptides detected with Anti-biotin (D5A7) Rabbit mAb at different concentrations. TMB substrate #7004 was used for color development.ELISA结果显示采用不同浓度抗生物素（D5A7）兔源单克隆抗体检测的生物素化和为生物素化的肽段在450nm处的吸光值。所用底物为TMB substrate #7004。 | |
ELISA using Anti-biotin (D5A7) Rabbit mAb coated plates to capture biotinylated phospho and non-phosphopeptides. Peptide detection was performed using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 followed by Anti-mouse IgG, HRP-linked Antibody #7076. TMB Substrate #7004 was added for color development.采用抗生物素（D5A7）兔源单克隆抗体包被板子，捕获生物素化的二氧磷基和无二氧磷基的肽段进行ELISA分析。肽段的检测采用Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 和Anti-mouse IgG, HRP-linked Antibody #7076。所用底物为TMB substrate #7004。 | |
Western Blot analysis of extracts from HeLa and NIH/3T3 cells using S6 Ribosomal Protein (54D2) Mouse mAb (Biotinylated) #3944 followed by Anti-biotin (D5A7) Rabbit mAb and anti-rabbit IgG, HRP linked Antibody #7074.Western blot分析HeLa 和 NIH/3T3 细胞的提取物，所用抗体为S6 Ribosomal Protein (54D2) Mouse mAb (生物素化d) #3944 和Anti-biotin (D5A7) Rabbit mAb and anti-rabbit IgG, HRP linked Antibody #7074. |
