| 货号 | 3999S |
| 反应种属 | All |
| 应用 | W |
| 供应商 | CST |
| 背景 | Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins (1,2). Chemiluminescent detection systems have emerged as the best all-around detection method for use with western blots and ELISA. These detection assays eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives. Streptavidin-HRP is used with biotinylated proteins and specific chemiluminescent substrates to generate light signal. Streptavidin-HRP conjugates have a very high turnover rate, coupling high sensitivity with short reaction times. |
| 存放说明 | -20C |
| 参考文献 | 1 . Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95. 2 . Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72. 3 . Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5. 4 . Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30. |
Western blot analysis of extracts from HeLa cells, untreated or treated with Paclitaxel #9807, using Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 and Streptavidin-HRP #3999 for detection.Western blot 分析HeLa细胞，未转染组和采用Paclitaxel #9807的转染组，所用抗体为Phospho-Histone H3 (Ser10) (D2C8) Rabbit mAb (Biotinylated) #3642 ，检测用抗体为Streptavidin-HRP #3999 |
