| 货号 | 5947S |
| 应用 | IP |
| 供应商 | CST |
| 标记 | Magnetic Bead |
| 背景 | Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2). |
| 存放说明 | 4C |
| 参考文献 | 1 . Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95. 2 . Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72. 3 . Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5. 4 . Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30. |
Immunoprecipitation of phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from NIH/ 3T3 cell extracts, treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (10 min), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Biotinylated) #4094 and Streptavidin (Magnetic Bead Conjugate) (lane 3). Lysate and Streptavidin (Magnetic Bead Conjugate) are shown in lane 2, demonstrating streptavidin bead binding. Lane 1 shows 10% lysate. Western blot analysis was performed using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106.对NIH/ 3T3细胞提取物中的phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)进行免疫共沉淀，用phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)处理（10分钟），使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Biotinylated) #4094和Streptavidin (Magnetic Bead Conjugate) (泳道3)。细胞裂解液和磁珠绑定的链霉亲和素如泳道2所示，表明链霉亲和素的结合能力。泳道1为10%的上样。Western blot分析，使用的抗体是Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106。 |
