Goat Polyclonal Goat anti-Rat IgG Fc Secondary Antibody [DyLight 649] (Pre-absorbed)
货号：
NBP1-51886 基本售价：
4045.1 元 规格：
-
产品信息
概述| 货号 | NBP1-51886 |
| 克隆类别 | Polyclonal |
| 同种亚型 | IgG |
| 反应种属 | Rat |
| 来源宿主 | Goat |
| 应用 | Flow, ICC/IF, IHC, IHC-P |
| 目标/特异性 | Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab)2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight 649. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and mouse IgG was detected. This antibody may cross react with IgG from other species. |
性能| 供应商 | Novus |
| 免疫原 | Rat IgG-Fc Fragment |
| 存放说明 | Store at 4C. Do not freeze. |
| 存储溶液 | PBS containing 0.2% BSA |
| 浓度 | 0.5 mg/ml |
| 纯度 | Immunogen affinity purified |
