| 货号 | AHP949AB |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Sheep |
| 来源宿主 | Rabbit |
| 应用 | E |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Rabbit anti Sheep IgA antibody used as a capture reagent (AHP949) together with horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) as a detection reagent for the measurement of total IgA in sheep saliva using a sandwich ELISA. Image caption: Stability of total sheep IgA (A) and IgG (B) (n = 15), rGT2-specific IgA (C) and IgG (D) (n = 10) exposed to rumen contents from sheep. Saliva was added to rumen contents with or without addition of protease inhibitor or added to PBS. Ig concentrations were measured by ELISA. Data are presented as mean (± SE) proportion of antibody relative to levels at time 0. Significant differences to time 0 were shown as *P <0.05, **P <0.001. From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016) Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens. PLoS ONE 11(7): e0159861. | |
Published customer image: Horseradish peroxidase conjugated Rabbit anti Sheep IgA antibody (AHP949P) used as a detection reagent for the measurement of total IgA in sheep saliva by western blotting. Image caption: Western blot analysis showing the stability of sheep IgA (left) and IgG (right) before (0 h) and after (4 h) incubation with sheep rumen contents or PBS at 39°C. Lane 1: standards of different molecular weights; lane 2: rumen contents (0 h); lane 3: rumen contents (4 h); lane 4: rumen contents with protease inhibitor (0 h); lane 5: rumen contents with protease inhibitor (4 h); lane 6: PBS (0 h) and lane 7: PBS (4 h). From: Subharat S, Shu D, Zheng T, Buddle BM, Kaneko K, Hook S, et al. (2016) Vaccination of Sheep with a Methanogen Protein Provides Insight into Levels of Antibody in Saliva Needed to Target Ruminal Methanogens. PLoS ONE 11(7): e0159861. | |
Published customer image: Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique. Image caption: Scatterplots showing relationships among the four fecal antibody measures (“anti-Tc”: anti-T. circumcincta antibodies). X-axis and y-axis units represent optical densities at a wavelength of 450 nm. From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN. Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies. Ecol Evol. 2015 Dec 8;6(1):56-67. | |
Published customer image: Relationship between fecal and plasma antibody measurements. Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique. Image caption: Scatterplots showing relationships between the four fecal antibody measures and the five plasma antibody measures (“anti-Tc”: anti-T. circumcincta antibodies), with points in red where the correlation was statistically significant. X-axis and y-axis units represent optical densities at a wavelength of 450 nm. From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN. Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies. Ecol Evol. 2015 Dec 8;6(1):56-67. | |
Published customer image: Antibody response by age and sex group. Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique. Image caption: Variation in the four fecal and five plasma antibody measures with respect to age and sex group. Each data point is represented by a black dot. For box plots, the bar in centre of box represents the median value, the box represents the interquartile range, and bars indicate the 5–95th percentile range. fem, female. From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN. Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies. Ecol Evol. 2015 Dec 8;6(1):56-67. | |
Published customer image: Antibody response by Fecal egg count according to age and weight. Rabbit anti Sheep IgA antibody(AHP949) used as a capture reagent for onvine IgA in a sandwich ELISA along with horseradish peroxidase conjugated Rabbit and Sheep IgA antibody (AHP949P) as a detection reagent to measure total fecal IgA in ovine samples and, with mouse monoclonal anti T. circumcincta antibody, clone 2F1 together with horseradish peroxidase conjugated Goat anti Moiuse IgG1 antibody (STAR132P) to detect parasite specific IgA response, also using a sandwich ELISA technique. Image caption: Variation in weight (A) and log-transformed strongyle fecal egg count (FEC) with respect to age and sex groups, with the mean and SEM indicted by the bars. (C, D): Scatterplot illustrating the significant negative correlation between FEC and fecal anti-T. circumcincta IgA and IgG antibody levels, respectively. From: Watt KA, Nussey DH, Maclellan R, Pilkington JG, McNeilly TN. Fecal antibody levels as a noninvasive method for measuring immunity to gastrointestinal nematodes in ecological studies. Ecol Evol. 2015 Dec 8;6(1):56-67. |
