| 货号 | F0113 |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 同种亚型 | IgG |
| 反应种属 | Rat |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects rat IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate mouse cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab)2 fragments which have reduced interactions with cell surface Fc receptors. |
| 使用方法 | Flow Cytometry: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of rat origin, and is of IgG class. The recommendedconcentration is 10 μL/106cells. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Rat IgG heavy and light chains |
| 生物活性 | Rat |
| 标记 | Allophycocyanin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat IgGPrimary Antibody by Flow Cytometry bEnd.3 mouse endothelioma cell line was stained with RatAnti-Mouse Neuropilin-1 Monoclonal Antibody (Catalog # MAB5994,filled histogram) or isotype control antibody (Catalog # MAB006),open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113) at 10 μL/106cells. |
