| 货号 | F0104B |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 同种亚型 | IgG |
| 反应种属 | Rat |
| 应用 | Flow Cytometry |
| 目标/特异性 | Rat IgG specific IgG was purified by rat IgG affinity chromatography. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Rat IgG heavy and light chains |
| 生物活性 | Rat |
| 标记 | Fluorescein |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat IgGPrimary Antibody by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line wasstained with Rat Anti-Mouse IL‑38/IL‑1F10 Monoclonal Antibody (Catalog #MAB7774,filled histogram) or isotype control antibody (open histogram), followedby Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B)at 10 uL/106 cells. Tofacilitate intracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin. |
