货号 | HAF005 |
别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
同种亚型 | IgG |
反应种属 | Rat |
应用 | Western Blot,Simple Western |
目标/特异性 | Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed. |
使用方法 | Western Blot: Use at a 1:1000 dilution. Simple Western: Use at a 1:50 dilution. |
来源 | Polyclonal Goat IgG |
产品组分 |
供应商 | R&D Systems |
应用文献 | |
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes. | |
纯化方式 | Antigen Affinity-purified |
免疫原 | Rat IgG |
生物活性 | Rat |
标记 | HRP |
背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of Rat IgGPrimary Antibody by Western Blot Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a MonoclonalAntibody (Catalog # MAB645)using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1. | |
Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of RatAnti-Mouse S100A9 Monoclonal Antibody (Catalog #MAB2065)using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions andusing the 12-230 kDa separation system. |