| 货号 | F0126 |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 同种亚型 | IgG |
| 反应种属 | Sheep |
| 应用 | Flow Cytometry |
| 目标/特异性 | Sheep IgG specific IgG was purified by sheep IgG (H+L) affinity chromatography and absorbed against human IgG, and human, mouse, bovine, and rabbit serum to eliminate cross-reactivity. |
| 使用方法 | Flow Cytometry: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of sheep origin, and is of IgG class. The recommended concentration is 10 μL/106cells. |
| 来源 | Polyclonal Donkey IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Sheep IgG |
| 生物活性 | Sheep |
| 标记 | Phycoerythrin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of SheepIgG Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line wasstained with Sheep Anti-Human AHR Antigen Affinity-purified Polyclonal Antibody (Catalog #AF6185,filled histogram) or isotype control antibody (Catalog # 5-001-A,open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG SecondaryAntibody (Catalog # F0126) at 10 μL/106 cells. To facilitateintracellular staining, cells were fixed with paraformaldehyde andpermeabilized with saponin. |
