| 货号 | IC016N |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 应用 | Intracellular Staining by Flow Cytometry() |
| 目标/特异性 | Serum was obtained from naive (non-immunized) sheep. |
| 使用方法 | Control: Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 5 μL/106cells. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Protein A or G purified |
| 免疫原 | NA |
| 标记 | Alexa Fluor 700 |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of SheepIgG Control by Flow Cytometry Human peripheral blood monocytes were stained with SheepAnti-Human IRF5 Alexa Fluor® 700‑conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC4508N,filled histogram) or Sheep IgG Alexa Fluor® 700-conjugatedIsotype Control Antibody (Catalog # IC016N, open histogram). Tofacilitate intracellular staining, cells were fixed with Flow CytometryFixation Buffer (Catalog # FC004)and permeabilized with Flow CytometryPermeabilization/Wash Buffer I (Catalog # FC005). View our protocol for StainingIntracellular Molecules. |
