| 货号 | IC016A |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 应用 | Control() |
| 目标/特异性 | Serum was obtained from naive (non-immunized) sheep. |
| 使用方法 | Control: Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106cells. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | NA |
| 标记 | Allophycocyanin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Sheep IgGControl by Flow Cytometry HepG2human hepatocellular carcinoma cell line was stained with SheepAnti-Human/Mouse Arginase 1/ARG1 APC-conjugated Antigen Affinity-purifiedPolyclonal Antibody (Catalog # IC5868A,filled histogram) or Sheep IgG APC-conjugated Isotype Control Antibody(Catalog # IC016A, open histogram). To facilitate intracellularstaining, cells were fixed with Flow Cytometry Fixation Buffer(Catalog # FC004)and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I(Catalog # FC005).View our protocol for StainingIntracellular Molecules. |
