Donkey Anti-Sheep IgG HRP Affinity Purified PAb (1 ML)【科瑞奥博】

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Donkey Anti-Sheep IgG HRP Affinity Purified PAb (1 ML)

货号： HAF016 基本售价： 1407.8 元规格： -

产品信息

概述
货号	HAF016
别名	immunoglobulin heavy constant gamma 1 (G1m marker)
全称	Immunoglobulin G
同种亚型	IgG
反应种属	Sheep
应用	Western Blot,Simple Western
目标/特异性	Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.
使用方法	Western Blot: Use at a 1:1000 dilution. Simple Western: Use at a 1:50 dilution.
来源	Polyclonal Donkey IgG
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis. Authors: Bose C, Megyesi J, Shah S, Hiatt K, Hall K, Karaduta O, Swaminathan S PLoS ONE, 2015;10(8):e0136563. Species: Human Sample Type: Cell Lysates Application: WB A Novel Locus Harbouring a Functional CD164 Nonsense Mutation Identified in a Large Danish Family with Nonsyndromic Hearing Impairment. Authors: Nyegaard M, Rendtorff N, Nielsen M, Corydon T, Demontis D, Starnawska A, Hedemand A, Buniello A, Niola F, Overgaard M, Leal S, Ahmad W, Wikman F, Petersen K, Cruger D, Oostrik J, Kremer H, Tommerup N, Frodin M, Steel K, Tranebjaerg L, Borglum A PLoS Genet, 2015;11(7):e1005386. Species: Human Sample Type: Cell Lysates A Cre-conditional MYCN-driven neuroblastoma mouse model as an improved tool for preclinical studies. Authors: Althoff K, Beckers A, Bell E, Nortmeyer M, Thor T, Sprussel A, Lindner S, De Preter K, Florin A, Heukamp L, Klein-Hitpass L, Astrahantseff K, Kumps C, Speleman F, Eggert A, Westermann F, Schramm A, Schulte J Oncogene, 2015;34(26):3357-68. Species: Human Sample Type: Tissue Homogenates Application: WB Bacterial delivery of Staphylococcus aureus alpha-hemolysin causes regression and necrosis in murine tumors. Authors: St Jean A, Swofford C, Panteli J, Brentzel Z, Forbes N Mol Ther, 2014;22(7):1266-74. Species: Bacteria - E. Coli Sample Type: Cell Lysates Application: WB Structure-function analysis of Nel, a thrombospondin-1-like glycoprotein involved in neural development and functions. Authors: Nakamura R, Nakamoto C, Obama H J. Biol. Chem., 2012;287(5):3282-91. Species: Sheep Sample Type: IgG Application: WB Nkx3.2 promotes primary chondrogenic differentiation by upregulating Col2a1 transcription. Authors: Kawato Y, Hirao M, Ebina K, Shi K, Hashimoto J, Honjo Y, Yoshikawa H, Myoui A PLoS ONE, 2012;7(4):e34703. Species: Mouse Sample Type: Cell Lysates Application: WB Neurocan contributes to the molecular heterogeneity of the perinodal ECM. Authors: Bekku Y, Oohashi T, Arch. Histol. Cytol., 2010;73(2):95-102. Species: Mouse Sample Type: Tissue Homogenates
纯化方式	Antigen Affinity-purified
免疫原	Sheep IgG
生物活性	Sheep
标记	HRP
背景	R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
运输条件	Blue Ice
存放说明	4℃
参考文献	New Tools: NorthernLights New Fluorescent Secondary Antibodies

参考图片

Detection of SheepIgG Primary Antibody by Western Blot Western blot shows detection of Sheep Anti-Human/Mouse/Rat PSMA1Antigen Affinity-purified Polyclonal Antibody
(Catalog #AF7565)using a 1:1000 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody(Catalog # HAF016). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Detection of Sheep IgG Primary Antibody by Simple Western^TM. Simple Western lane view shows detection of Sheep Anti-Mouse Contactin-6 Polyclonal Antibody
(Catalog #AF5890) using a 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.