| 货号 | AAI28 |
| 同种亚型 | Polyclonal IgG |
| 反应种属 | Chicken |
| 来源宿主 | Goat |
| 应用 | E, ID |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | |
| 存放说明 | Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
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Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgA antibody (AAI28P) used for the measurement of IgA levels by ELISA. Image caption: Antibody levels in APEC- and mock-infected birds following primary infection and after homologous re-challenge. The levels were measured by ELISA using different antigens; IgY levels against soluble χ7122nalR antigens (A), IgY levels against O78 LPS purified from χ7122nalR (B) and IgA levels measured against soluble χ7122nalR antigens (C). At 0, 7, 14, 21, and 28 days post-primary infection, five birds per group were sampled and ten birds per group were sampled after re-challenge (day 42). The data in panels B and C reflect levels at post-mortem examination on day 42, one day after re-challenge. *?=?P?≤?0.05; **?=?P?≤?0.01. From: Sadeyen JR, Kaiser P, Stevens MP, Dziva F. Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge. Vet Res. 2014 45:19. | |
Published customer image: Alkalinephosphatase conjugated Goat anti Chicken IgA antibody used for the evaluation of IgA levels in chickens by ELISA Image caption: Antigen-specific serum IgM (A), IgA (B), and IgG (C) against S. Virchow and S. Typhimurium at 5, 11, and 26 DPI, following infection of 7-day old chickens. Data shown are based on five birds per group at each time point. Error bars represent the standard error of the mean. An asterisk indicates that there was a significant difference between the S. Virchow infected group and uninfected group (*) or the S. Typhimurium infected group and the uninfected group (**) (P < 0.05). From: Salisbury A-M, Leeming G, Nikolaou G, Kipar A and Wigley P (2014) Salmonella Virchow infection of the chicken elicits cellular and humoral systemic and mucosal responses, but limited protection to homologous or heterologous re-challenge. Front. Vet. Sci. 1:6. | |
Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgA antibody used for the evaluation of flagellin specific IgA response in exposed mice by ELISA. Image caption: Antigen-specific serum antibody responses. NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. | |
Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed mice by ELISA. Image caption: Kinetics of NHC-specific antibody levels following in ovo vaccination. ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. | |
Published customer image: Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed mice by ELISA. Image caption: Cecal colonization of C. jejuni and sIgA responses in vaccinated and control chickens.(A) Chickens were challenged with C. jejuni at 18 day of age. One week post-inoculation (25 days of age) the number C. jejuni in the ceca was determined by plate counting (CFU/ g). Each symbol represents one chicken of the indicated groups, bars represent the mean number of log10 CFU/ g per group. (B) Antigen-specific sIgA in 20-times diluted cecal content from 25 day-old chickens (one week after C. jejuni challenge) as determined by ELISA. Graphs represent the analysis of individual animals of each of the indicated groups, displayed as a box-and-whisker plot. From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni. PLoS ONE 11(10): e0164837. |
