货号 | STAR36D488GA |
同种亚型 | Polyclonal IgG |
反应种属 | Rabbit |
来源宿主 | Sheep |
应用 | F, IF, WB |
供应商 | Bio-Rad Antibodies |
运输条件 | |
存放说明 | Store at +4oC or at -20oC if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4oC or at -20oC if preferred. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
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HeLA cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using AHP2167, a Rabbit polyclonal anti-Human Microtubule associated protein 1LC3A/B (MAP1LC3A/B) at 1/25. STAR36D488GA, Sheep anti-Rabbit DyLight®488 was then applied at 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification. | |
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green | |
Dylight®649 conjugated Sheep anti Rabbit IgG antibody (STAR36D649) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Bound Mouse anti Human PCNA antibody (MCA1558 ) was simultaneously detected with Dylight®549) conjugated Goat anti Mouse IgG antibody (STAR117D549GA) | |
Dylight®800 conjugated Sheep anti Rabbit antibody (STAR36D800) used to detect bound Rabbit anti Human GAPDH antibody (AHP1628). Cyclophilin A was detected using Dylight®680 conjugated Human anti Human cyclophilin A antibody (HCA005D680) | |
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®488 (STAR117D488GA) shown in green and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®680 (STAR36D680GA) shown in red |