Donkey Anti-Goat IgG HRP Affinity Purified PAb (1 ML)【科瑞奥博】

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Donkey Anti-Goat IgG HRP Affinity Purified PAb (1 ML)

货号： HAF109 基本售价： 1407.8 元规格： -

产品信息

概述
货号	HAF109
别名	immunoglobulin heavy constant gamma 1 (G1m marker)
全称	Immunoglobulin G
同种亚型	IgG
反应种属	Goat
应用	Western Blot,Simple Western
目标/特异性	Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with human IgG, rabbit IgG, mouse IgG and less than 1% cross-reactivity with chicken IgY is observed.
使用方法	Western Blot: Use at a 1:1000 dilution. Simple Western: Use at a 1:50 dilution.
来源	Polyclonal Donkey IgG
产品组分

性能
供应商	R&D Systems
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Rebalancing gene haploinsufficiency in vivo by targeting chromatin Authors: Filomena Gabriella Fulcoli Nat Commun, 2016;7(0):11688. Species: Mouse Sample Type: Whole Cells Application: WB Involvement of a C-terminal motif in the interference of primate lentiviral Vpu proteins with CD1d-mediated antigen presentation. Authors: Bachle S, Sauter D, Sibitz S, Sandberg J, Kirchhoff F, Moll M Sci Rep, 2015;5(0):9675. Species: Human Sample Type: Cell Lysates Application: Bioassay Nrf2-inducing anti-oxidation stress response in the rat liver--new beneficial effect of lansoprazole. Authors: Yamashita, Yasunobu, Ueyama, Takashi, Nishi, Toshio, Yamamoto, Yuta, Kawakoshi, Akatsuki, Sunami, Shogo, Iguchi, Mikitaka, Tamai, Hideyuki, Ueda, Kazuki, Ito, Takao, Tsuruo, Yoshihir, Ichinose, Masao PLoS ONE, 2014;9(5):e97419. Species: Rat Sample Type: Tissue Homogenates Application: WB Oxidative stress induces mitochondrial dysfunction in a subset of autism lymphoblastoid cell lines in a well-matched case control cohort. Authors: Rose, Shannon, Frye, Richard, Slattery, John, Wynne, Rebecca, Tippett, Marie, Pavliv, Oleksand, Melnyk, Stepan, James, S Jill PLoS ONE, 2014;9(1):e85436. Species: Human Sample Type: Cell Lysates Application: WB Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells. Authors: Lin CE, Chen SU, Lin CC PLoS ONE, 2012;7(7):e41096. Species: Human Sample Type: Cell Lysates Application: WB Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression. Authors: Furukawa K, Sato T, Katsuno T, Nakagawa T, Noguchi Y, Tokumasa A, Yokote K, Yokosuka O, Saito Y Biochem. Biophys. Res. Commun., 2011;405(4):521-6. Species: Mouse Sample Type: Cell Lysates Application: WB Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation. Authors: Faller EM, Sugden SM, McVey MJ J. Immunol., 2010;185(5):2854-66. Species: Human Sample Type: Cell Lysates Application: WB BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D PLoS ONE, 2010;5(9):e12995. Species: Mouse Sample Type: Tissue Lysate Application: WB Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Authors: Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Cancer Res., 2009;69(7):2996-3003. Species: Human Sample Type: Cell Lysates Application: WB Contribution of globular death domains and unstructured linkers to MyD88.IRAK-4 heterodimer formation: an explanation for the antagonistic activity of MyD88s. Authors: Mendoza-Barbera E, Corral-Rodriguez MA, Soares-Schanoski A, Velarde M, Macieira S, Messerschmidt A, Lopez-Collazo E, Fuentes-Prior P Biochem. Biophys. Res. Commun., 2009;380(1):183-7. Species: Human Sample Type: Cell Lysates Application: WB Cilostazol inhibits modified low-density lipoprotein uptake and foam cell formation in mouse peritoneal macrophages. Authors: Okutsu R, Yoshikawa T, Nagasawa M, Hirose Y, Takase H, Mitani K, Okada K, Miyakoda G, Yabuuchi Y Atherosclerosis, 2009;204(2):405-11. Species: Mouse Sample Type: Cell Lysates Application: WB Evaluation of an LC8-binding peptide for the attachment of artificial cargo to dynein. Authors: Bergen JM, Pun SH, Mol. Pharm., 2007;4(1):119-28. Species: Human Sample Type: IgG Application: ELISA Development Plasma storage at -80 degrees C does not protect matrix metalloproteinase-9 from degradation. Authors: Rouy D, Ernens I, Jeanty C, Wagner DR Anal. Biochem., 2005;338(2):294-8. Species: Human Sample Type: Plasma Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Goat IgG
生物活性	Goat
标记	HRP
背景	R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
运输条件	Blue Ice
存放说明	4℃
参考文献	New Tools: NorthernLights New Fluorescent Secondary Antibodies

参考图片

Detection of Goat IgGPrimary Antibody by Western Blot Western blot shows detection of Goat Anti-Human/Mouse/Rat GRP75/HSPA9BPolyclonal Antibody (Catalog # AF3584)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF109). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 2.

Detection of Goat IgG Primary Antibody by Simple Western^TM. Simple Western lane view shows detection of Goat Anti-Human/Mouse/Rat TC-PTP Polyclonal Antibody (Catalog #AF1930) using a 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.