| 货号 | PAP004 |
| 反应种属 | Chicken |
| 应用 | FN |
| 是否允许进口 | 否 |
| 是否危险品 | 是 |
| 供应商 | Bio-Rad Antibodies |
| 运输条件 | 干冰运输 |
| 存放说明 | Store at -20oC only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
Published customer image: Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus. Image caption: Recombinant ChIFN-a reduces avian influenza virus replication. Inhibition of avian influenza virus (H1N1 and H5N9) replication in primary lung cell cultures derived from chicken (A), duck (B), and turkey (C) after rChIFN-a (1000 U/ml) pretreatment in vitro. Cells were infected with A/turkey/Virginia/2009 H1N1 or A/turkey/Wisconsin/68 H5N9 at MOI 0.1. Supernatants were harvested at the times indicated and viral titers were determined following injection into SPF embryos. The mean (and standard deviations) of three independent experiments are shown. Different lowercase letters denote significance in titer following rChIFN-a treatment groups (within columns) (P < 0.05) as determined by one-way ANOVA. Statistical differences (P < 0.05) following treatment between virus groups are shown by lowercase letter. From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species. Virol J. 2011 Sep 22;8:447. | |
Published customer image: Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus. Image caption: Recombinant ChIFN-a inhibits pH1N1 and H5N9 virus nuclear protein expression. Primary chicken, turkey, and duck lung cells were pretreated with or without rChIFN-a (1000 U/ml) for 18 h. Monolayers were infected with either H1N1 or H5N9 avian influenza virus (MOI = 0.1) for 1 h, and replaced with fresh media. After 24 hours, cells were fixed and viral antigens were reacted with mouse-derived monoclonal antibody (P13C11) specific for type A influenza virus nucleoprotein followed by detection with Texas Red-labeled goat anti-mouse IgG antibody. Magnification 400×. From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species. Virol J. 2011 Sep 22;8:447. | |
Published customer image: Recombinant Chicken interferon alpha used to treat chicken, duck and turkey lung cells prior to infection by avian flu virus. Image caption: Reduced cytopathic effect following rChIFN-a pretreatment following AIV infection. Primary chicken lung cell monolayers were pretreated with 1000 U/ml of rChIFN-a and infected with either H1N1 of H5N9 at 0.1 MOI. Negative control cells include no treatment/no virus (A), and IFN-a only (B). Protection from cytopathic effect was observed in cells infected with virus only, H1N1 (C) and H5N9 (E), compared with IFN-a treated cells that were then infected with H1N1 (D) or H5N9 (F). At 24 hpi the monolayers were digitally photographed using an inverted microscope at 200× magnification (Olympus America Inc., Melville, NY). From: Jiang H, Yang H, Kapczynski DR. Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species. Virol J. 2011 Sep 22;8:447. | |
Published customer image: Recombinant chicken interferon alpha used to treat chicken DF-1 fibroblastoid cells prior to infection with avian influenza virus. Image caption: Chicken IFITM3 has an antiviral activity in DF-1 chicken cells. The expression level and log fold change of chIFITM3 were measured using quantitative RT-PCR after stimulation with IFN-a and IFN-? or after preincubation with a nontargeting siRNA or one specific to chIFITM3 (A). The effect of knocking down endogenous chIFITM3 expression in DF-1 cells infected with influenza A virus (A/WSN/1933 [WSN/33]) was measured by flow cytometry using an antibody against nucleoprotein (NP) (B). P = 0.01, Student's t test. DF-1 cells transfected with chIFITM3-HA were infected by WSN. Expression of HA and NP was detected by flow cytometry (C and D), and viral titers were measured by PFU (E). Error bars represent standard deviations across each condition performed in triplicate. From: Smith SE, Gibson MS, Wash RS, Ferrara F, Wright E, Temperton N, Kellam P, Fife M. Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssa viruses in vitro. JVirol. 2013 Dec;87(23):12957-66. |
