SHEEP ANTI BOVINE IgG:FITC【科瑞奥博】

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SHEEP ANTI BOVINE IgG:FITC

货号： AAI23F 基本售价： 2657.0 元规格： -

产品信息

概述
货号	AAI23F
同种亚型	Polyclonal IgG
反应种属	Bovine
来源宿主	Sheep
应用	C, F

性能
供应商	Bio-Rad Antibodies
运输条件
存放说明	Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.Store at +4^oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

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参考图片

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP-specific Ig titres following primary and boost immunisations with TNP-conjugated antigens. Five calves were subcutaneously immunised with 3 mg TNP-CGG or TNP-AECM-FICOLL in QuilA then boosted 29 days later with the same immunising antigens. The dotted vertical line indicates when the booster TNP-CGG/TNP-AECM-FICOLL immunisations were given (day 29 pi). Blood samples were taken at various time-points following these injections and TNP-specific IgG (diamond symbols) and IgM (square symbols) titres were determined by ELISA in calves from the T-D group (open symbols) or T-I group (filled symbols). Results are expressed as the mean of determinations from individual calves in each group?±?SEM, n?=?5.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
TNP-specific plasma and memory B cell detection post-boost immunisation with TNP-CGG (T-D group). Five calves were subcutaneously immunised with 3 mg TNP-CGG in QuilA then boosted 29 days later with the same immunising antigen. The dotted vertical line indicates when the booster TNP-CGG immunisation was given (day 29 pi). Blood samples were taken at various time-points following the immunisations. TNP-specific IgG, plasma cell (filled circles, left-axis) and memory B cell (open circles, right-axis) numbers were determined by B cell ELISPOT. Results were expressed as ASC per 10⁶ PBMC for plasma cells and induced ASC per 10⁶ cultured PBMC for memory B cells (mean of determinations from individual calves in each group, n?=?5).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
Kinetics of antigen-specific IgG secreting plasma cells and memory B cells post-TTC immunisation. At day 134 post-primary TNP-conjugated immunisation, all calves were subcutaneously immunised with 3 mg TTC in IFA then boosted 63 days later with the same immunising antigen. Blood samples were taken at various time-points following these immunisations. The time of primary and boost TTC immunisations are indicated by vertical dotted lines (day 134 and 197 pi, respectively). Antigen-specific IgG, plasma cell (A) and memory B cell (B) numbers were determined by B cell ELISPOT and are identified as follows: CGG-specific (open triangle), TTC-specific (crosses) and TNP-specific (closed triangle) IgG secreting ASCs. Results were expressed as ASC per 10⁶ PBMC for plasma cells and induced ASC per 10⁶ cultured PBMC for memory B cells (mean of determinations from individual calves in each group for the TNP- and CGG-specific responses, n?=?5, and mean of determinations from all calves for the TTC-specific responses, n?=?10). a?=?p?<?0.05, b?=?p?<?0.001.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP- and TTC-specific IgG titres following primary and boost TTC immunisations. TNP-specific IgG titres in calves from the T-D group (open diamonds) or T-I group (filled diamonds) and TTC-specific IgG titres in all calves (cross symbols) were determined by ELISA. The dotted vertical lines indicate when the primary and boost TTC immunisations were administered (days 134 and 197 pi, respectively). Results are expressed as the mean of determinations from individual calves in each group for the TNP-specific responses (n?=?5) and the mean of determinations from all calves for the TTC-specific responses (n?=?10).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the measurement of cattle IgG resopnses totsetse fly antigens by ELISA.
Image caption:
Cattle IgG responses against WSE and, Tsal152–75 and Tsal1145–166 peptides. The IgG responses directed against whole saliva extracts (WSE) and the two candidate synthetic peptides were investigated in 43 animals from a tsetse infested area, 17 animals from a tsetse free area and four animals exposed experimentally to A. variegatum, An. gambiae, Tabanidae spp.orStomoxys spp. (other arthropods). Individual =ΔOD values are represented by empty circles. In the scatter plot, the horizontal bars indicate the median value for each group. Statistical significance between the different groups is indicated (non-parametric Mann–Whitney U-test)

From: Somda MB, Cornelie S, Bengaly Z, Mathieu-Daudé F, Poinsignon A, Dama E, Bouyer J, Sidibé I, Demettre E, Seveno M, Remoué F, Sanon A, Bucheton B.
Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
Parasit Vectors. 2016 Mar 15;9(1):149.

Published customer image:
Biotin conjugated Sheep anti Bovine IgG antibody (AAI23B) used for the detection of M. aviumsubsp.paratuberculosis protein specific IgG secreted by cells from infected calves by ELISPOT along with biotinylated Sheep anti Bovine IgA antibody (AAI20B) used to evaluate similar IgA responses.
Image caption:
Net IgA-ASC and IgG-ASC responses to recombinant M. aviumsubsp.paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. aviumsubsp.paratuberculosis-infected and uninfected compartments in mid-jejunal segments and assayed for IgA (A) and IgG (B) ASCs specific to recombinant M. aviumsubsp.paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. aviumsubsp.paratuberculosis-infected and uninfected compartments in terminal small intestinal segments and assayed for IgG (C) ASCs specific to recombinant M. aviumsubsp.paratuberculosis proteins. The net number of ASC specific to each M. aviumsubsp.paratuberculosis protein in infected compartments was calculated using the formula provided in Materials and Methods. M. aviumsubsp.paratuberculosis proteins for which all calves (n = 3) had detectable net ASC responses are shown with significant (P <0.05) increases in the frequency of M. avium subsp. paratuberculosis-protein specific ASCs denoted by an asterisk. Data presented are mean values with SEM.

From: Facciuolo A, Gonzalez-Cano P, Napper S, Griebel PJ, Mutharia LM (2016)
Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer’s Patches to Mycobacterium aviumsubsp.paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
>PLoS ONE 11(7): e0158747.