| 货号 | 15048 |
| 同种亚型 | IgG |
| 反应种属 | Rabbit |
| 来源宿主 | Goat |
| 使用方法 | Application: Immunofluorescence: 1:1,000 to 1:2,000 ATTO 647N (STED/GSD) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leicas STimulatedEmissionDepletion(STED) microscopes. It is also recommended by Leica for use in GroundStateDepletion with IndividualMolecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.  Absorption and emission spectra of ATTO 647N (STED/GSD) Dye.  Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody. HeLa cells stained with Histone H3 trimethyl Lys27 rabbit polyclonal antibody (Cat. No. 39155) at 1:500 dilution and ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody at 1:1,000. Green: Nucleolin mouse monoclonal antibody (Cat. No. 39541).  Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527)andChromeo 494 Goat anti-Mouse IgG (Cat. No. 15032) secondary antibody. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody. This STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Blue Ice |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 250 µl (Catalog No. 15048) or 35 µl (Catalog No. 15068) of affinity purified goat anti-rabbit IgG (H+L) conjugated to ATTO 647N (STED/GSD) in PBS, pH 7.4, 0.01% sodium azide. Specificity: This antibody wa |
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Absorption and emission spectra of ATTO 647N (STED/GSD) Dye. | |
Immunofluorescence staining using the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody. HeLa cells stained with Histone H3 trimethyl Lys27 rabbit polyclonal antibody (Cat. No. 39155) at 1:500 dilution and ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody at 1:1,000. Green: Nucleolin mouse monoclonal antibody (Cat. No. 39541). | |
Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 494 Goat anti-Mouse IgG (Cat. No. 15032) secondary antibody. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG secondary antibody. This STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
