| 货号 | 15044 |
| 同种亚型 | IgG |
| 反应种属 | Rabbit |
| 来源宿主 | Goat |
| 使用方法 | Applications: Immunofluorescence: 1:1,000 to 1:2,000 dilution Plate-based assays: 1:800 to 1:1,000 dilution  Absorption and emission spectra of Chromeo 642 Dye.  Chromeo 642 staining of etoposide-treated HeLa cells. HeLa cells were treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117)andChromeo 642 Goat anti-rabbit IgG.  Concurrent, multi-color staining of Phospho H2AX and tubulin using Chromeo 642 and Chromeo 488 secondary antibodies in untreated and etoposide-treated HeLa cells. HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117)andChromeo 642 Goat anti-rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2)andChromeo 488 Goat anti-mouse IgG (Cat. No. 15031). The nuclei were counter-stained with DAPI, then the three separate images were merged. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Room Temp |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 1 mg (Catalog No. 15044) or 0.1 mg (Catalog No. 15064) of Chromeo™ 642 conjugated Goat anti-Rabbit IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM so |
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Absorption and emission spectra of Chromeo 642 Dye. | |
Chromeo 642 staining of etoposide-treated HeLa cells. HeLa cells were treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG. | |
Concurrent, multi-color staining of Phospho H2AX and tubulin using Chromeo 642 and Chromeo 488 secondary antibodies in untreated and etoposide-treated HeLa cells. HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG (Cat. No. 15031). The nuclei were counter-stained with DAPI, then the three separate images were merged. |
