| 货号 | 15042 |
| 同种亚型 | IgG |
| 反应种属 | Rabbit |
| 来源宿主 | Goat |
| 使用方法 | Applications: Immunofluorescence: 1:100 to 1:250 dilution Plate-based assays: 1:800 to 1:1,000 dilution To ensure a maximum in photostability of Chromeo 494 conjugates under all experimental conditions, we recommend the use of Mowiol or TDE as mounting medium. Vectashield or MAXfluor Mounting medium might negatively influence the photostability of Chromeo 494. Chromeo 494 Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leicas STimulatedEmissionDepletion(STED) microscopes. STED enables resolution of 50-70 nm.  Absorption and emission spectra of Chromeo 494 Dye.  HeLa cells stained with alpha Tubulin pAb and Chromeo 494 Goat anti-Rabbit IgG. HeLa cells were stained with alpha Tubulin rabbit pAb and Chromeo 494 Goat anti-Rabbit IgG.  Multi-color staining of Phospho H2AX and tubulin using Chromeo 494 and Chromeo 488 secondary antibodies in untreated and etoposide-treated HeLa cells. HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117)andChromeo 494 Goat anti-Rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2, Cat. No. 39527)andChromeo 488 Goat anti-Mouse IgG (Cat. No. 15031). The nuclei were counter-stained with DAPI, then the three separate images were merged. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Room Temp |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 1 mg (Catalog No. 15042) or 0.1 mg (Catalog No. 15062) of Chromeo™ 494 conjugated Goat anti-Rabbit IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM so |
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Absorption and emission spectra of Chromeo 494 Dye. | |
HeLa cells stained with alpha Tubulin pAb and Chromeo 494 Goat anti-Rabbit IgG. HeLa cells were stained with alpha Tubulin rabbit pAb and Chromeo 494 Goat anti-Rabbit IgG. | |
Multi-color staining of Phospho H2AX and tubulin using Chromeo 494 and Chromeo 488 secondary antibodies in untreated and etoposide-treated HeLa cells. HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 494 Goat anti-Rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 488 Goat anti-Mouse IgG (Cat. No. 15031). The nuclei were counter-stained with DAPI, then the three separate images were merged. |
