| 货号 | 15040 |
| 同种亚型 | IgG |
| 反应种属 | Rabbit |
| 来源宿主 | Goat |
| 使用方法 | Applications: Immunofluorescence: 1:1,000 to 1:2,000 dilution Plate-based assays: 1:800 to 1:1,000 dilution Chromeo 505 Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leicas STimulatedEmissionDepletion(STED) microscopes. It has also been tested and verified by Leica for use in GroundStateDepletion with IndividualMolecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.  Absorption and emission spectra of Chromeo 505 Dye.  Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527), a biotinylated secondary goat anti-mouse antibody and BD Horizon V500-streptavidin conjugate. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Catalog No. 39159)andChromeo 505 Goat anti-Rabbit IgG secondary antibody. The STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Room Temp |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 1 mg (Catalog No. 15040) or 0.1 mg (Catalog No. 15060) of Chromeo™ 505 conjugated Goat anti-Rabbit IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM so |
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Absorption and emission spectra of Chromeo 505 Dye. | |
Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527), a biotinylated secondary goat anti-mouse antibody and BD Horizon V500-streptavidin conjugate. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Catalog No. 39159) and Chromeo 505 Goat anti-Rabbit IgG secondary antibody. The STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
