Mouse/Rabbit IgG VisUCyte HRP Polymer (25 ML)
货号：
VC002-025 基本售价：
2478.6 元 规格：
-
产品信息
概述| 货号 | VC002-025 |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) |
全称 | Immunoglobulin G | | 应用 | Immunohistochemistry()
|
| 目标/特异性 | Detects heavy ( gamma ) chains on mouse and rabbit IgG and light chains on all mouse and rabbit immunoglobulins, based on IEP. No cross-reactivity is observed with non-immunoglobulin mouse or rabbit serum proteins or serum proteins from bovine, horse, human, pig or rat, based on IEP. VisUCyte™ HRP Polymer provides high sensitivity with low background in IHC.
|
| 使用方法 | Immunohistochemistry: Prediluted, ready to use with formalin fixed paraffin-embedded and frozen sections. Compatible with most commonly used fixatives.
|
| 来源 | Polyclonal Goat IgG |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse and Rabbit IgG
|
| 生物活性 | Mouse, Rabbit |
| 标记 | HRP Polymer |
| 背景 | VisUCyte™ HRP Polymer is abiotin-free detection reagent which overcomes problems of avidin-biotindetection chemistry, such as endogenous biotin staining and the need to doadditional quenching of endogenous biotin and avidin present insome tissues. With VisUCyte HRP Polymer it is possible to achieve specific staining in cellsand tissue much faster than the conventional avidin-biotin-HRP procedure. Due the high sensitivity of VisUCyte™ HRP Polymer, the working concentration of primary antibodies maybe 2-3 times lower compared to the conventional avidin-biotin-HRP protocol. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - New Tools: NorthernLights New Fluorescent Secondary Antibodies
|
参考图片Detection of FABP1/L-FABP in Human Kidney. FABP1/L-FABP was detected in paraffin-embedded sections of human kidney using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB2964) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). |
Detection of Phospho-p38 (T180/Y182) in Mouse Embryo (13 d.p.c.). Phospho-p38 (T180/Y182) was detected in frozen sections of mouse embryo (13 d.p.c., cross-section through the gut) using Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAP Kinase (T180/Y182) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF869) at 1 μg/mL for 1 hour at room temperature followed by incubation for 30 minutes at room temperature with Anti-Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC002). Tissue was stained with DAB (brown color) and counterstained with hematoxylin (blue color). |
