Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate)
货号：
5127S 基本售价：
2194.0 元 规格：
-
产品信息
概述| 货号 | 5127S |
| 反应种属 | rabbit |
| 来源宿主 | Mouse |
| 应用 | W |
性能| 供应商 | CST |
| 标记 | HRP |
| 背景 | Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb only reacts with native IgG and does not bind to the denatured and reduced rabbit IgG heavy chain or light chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG light and heavy chains of the primary antibody used for IP run at approximately 25 and 50 kD, respectively, on the subsequent western blot and can often obscure bands of proteins that have similar molecular weights. Using Mouse Anti-Rabbit IgG (Conformation Specific) (L27A9) mAb as a secondary antibody will eliminate this problem. |
| 存放说明 | -20C |
参考图片Immunoprecipitation of PRAS40 from HeLa cells using PRAS40 (D23C7) XP® Rabbit mAb #2691 (lane 2). Lane 1 contains input control lysate. All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B). |
Immunoprecipitation of Beclin-1 from HeLa cells using Beclin-1 (D40C5) Rabbit mAb #3495 (lane 2). Lane 1 contains input control lysate. All lanes are probed with Beclin-1 (D40C5) Rabbit mAb #3495 as the primary antibody. Secondary antibodies include Anti-rabbit IgG, HRP-linked Antibody #7074 (panel A) and Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 (panel B).免疫沉淀分析HeLa细胞的Beclin-1 ，所用检测抗体为Beclin-1 (D40C5) Rabbit mAb #3495 (泳道2)，泳道1包含对照组的提取物。所由泳道采用Beclin-1 (D40C5) Rabbit mAb #3495 作为一抗。二抗包括Anti-rabbit IgG, HRP-linked Anti-body #7074 (图 A) 和 Mouse Anti-rabbit IgG (构象特异性) (L27A9) mAb (HRP 标记) #5127（ 图B）. |
