| 货号 | 3677S |
| 描述 | Affinity purified mouse anti-rabbit IgG (Light-Chain Specific) antibody. |
| 反应种属 | rabbit |
| 来源宿主 | Mouse |
| 应用 | W |
| 供应商 | CST |
| 背景 | Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunopreciptiation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-Rabbit IgG (Light-Chain Specific) (L57A3) mAb as a secondary antibody will eliminate this problem. |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Immunoprecipitation of PRAS40 from untreated HeLa cells using PRAS40 (D23C7) Rabbit mAb #2691 (Lane 2). All lanes are probed with PRAS40 (D23C7) Rabbit mAb #2691 as the primary antibody, including input control in lane 1. Secondary antibodies include Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), and Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound Mouse Anti-Rabbit IgG mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.免疫沉淀分析未处理组HeLa细胞的PRAS40 ，所用检测抗体为PRAS40 (D23C7) Rabbit mAb #2691 (泳道2)。所由泳道采用PRAS40 (D23C7) Rabbit mAb #2691作为一抗，泳道1中为对照。二抗包括Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mA(图 A) 和 Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb （ 图B） 和Anti-rabbit IgG, HRP-linked Antibody #7074 (图C)。对于图A和B， 采用Anti-mouse IgG, HRP-linked Antibody #7076可以检测到结合的mouse anti-rabbit IgG mAb ，可以显示出还原变性的兔IgG的位置。 | |
Western blot analysis of decreasing concentrations of total rabbit IgG reduced and denatured in 1X SDS loading buffer with DTT using Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 (Panel A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (Panel B), or Anti-rabbit IgG, HRP-linked Antibody #7074 (Panel C). For Panels A and B, the bound mouse anti-rabbit IgG mAb was detected by Anti-mouse IgG, HRP-linked Antibody #7076. The positions of the reduced and denatured rabbit IgG heavy and light chains are indicated.Western blot分析浓度递减的全兔IgG，采用带有DTT的SDS上样缓冲液使蛋白还原变性，所用检测抗体为Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 (图 A), Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb (图 B), 或 Anti-rabbit IgG, HRP-linked Antibody #7074 (图 C).对于图A和B， 采用Anti-mouse IgG, HRP-linked Antibody #7076可以检测到结合的mouse anti-rabbit IgG mAb ，可以显示出还原变性的兔IgG的位置。 |
