| 货号 | 4413S |
| 描述 | Anti-Rabbit IgG (H+L) F(ab)2 Fragment was conjugated to Alexa Fluor® 555 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab)2 fragment results in less non-specific binding to cells through Fc receptors. |
| 反应种属 | rabbit |
| 来源宿主 | Goat |
| 应用 | IF-IC |
| 使用方法 | IF-IC () |
| 供应商 | CST |
| 标记 | Alexa Fluor 555 |
| 背景 | Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence. |
Confocal immunofluorescent analysis of HeLa cells labeled with MEK1/2 (47E6) Rabbit mAb #9126 detected with Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 555 Conjugate) (red, left) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞，所用抗体为MEK1/2 (47E6) Rabbit mAb #9126检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (红色，上图)，与同型对照（下图）作比较。肌动蛋白丝采用fluorescein phalloidin (绿色)，蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084。 | |
Confocal immunofluorescent analysis of mouse cerebellum using α-Synuclein Antibody (IF Preferred) #2628 detected with Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 555 Conjugate) (red) and Neurofilament-L (DA2) Mouse mAb #2835 detected with Anti-Mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4408 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光分析鼠小脑细胞，所用抗体为α-Synuclein Antibody (IF Preferred) #2628检测抗体为anti- Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate) (红色)和Neurofilament-L (DA2) Mouse mAb #2835，检测抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 488 Conjugate) #4408 (绿色) 。蓝色伪彩为荧光DNA染料，信息为DRAQ5 ® #4084。 | |
High content analysis of A549 cells exposed to varying concentrations of LY294002 #9901 for 3 hrs, followed by 100 ng/mL EGF for 20 minutes. With increasing concentrations of LY294002, a significant decrease (~5 fold) in phospho-S6 Ribosomal Protein (Ser235/236) signal as compared to the uninhibited control was observed. When using phospho-S6 as a measurement, the IC50 of this compound was 3.06 μM. Data were generated on the Acumen HCS platform using Anti-Rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 555 Conjugate).图 高通量分析A549细胞，该细胞暴露于不同浓度的LY294002 #9901 3个小时。然后用100ng/mlde EGF 处理20分钟。与未抑制组相比较，随着LY294002 #9901浓度的增加phospho-S6 Ribosomal Protein (Ser235/236)信号显著降低（约5倍）。当phospho-S6 Ribosomal Protein 检测时，IC50为3.06mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 555 Conjugate)。 |
