| 货号 | 2965L |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 目标/特异性 | Phospho-Akt (Thr308) (C31E5E) Rabbit mAb detects endogenous levels of Akt only when phosphorylated at Thr308. |
| 使用方法 | WB(1:1000) F (1:100) IF-IC (1:1600) |
| 供应商 | CST |
| 背景 | Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19). |
| 存放说明 | -20C |
| 计算分子量 | 60 |
Confocal immunofluorescent analysis of C2C12 cells, insulin-treated (left) or LY294002-treated (right), using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测C2C12 细胞， 左图为胰岛素处理的 ，右图为LY294002处理的，检测抗体为Phospho-Akt (Thr308) (C31E5E) Rabbit mAb兔单抗，呈绿色。微丝用Alexa Fluor® 555 phalloidin标记（红色）。蓝色伪彩是 DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from NIH/3T3 and Jurkat cells, untreated, PDGF-treated or LY294002-treated as indicated, using Phospho-Akt (Thr308) (C31E5E) Rabbit mAb (upper) or Akt (pan) (C67E7) Rabbit mAb #4691 (lower). western blot方法检测细胞提取物：未处理和PDGF， LY294002处理的NIH/3T3 和 Jurkat细胞，使用的抗体为 Phospho-Akt (Thr308) (C31E5E) Rabbit mAb兔单抗 (上图) 和 Akt (pan) (C67E7)Rabbit mAb 兔单抗#4691 (下图). | |
Flow cytometric analysis of serum-starved NIH/3T3 cells, untreated (blue) or treated with mouse platelet-derived growth factor BB (200ng/ml, 10 min; green), using Phospho-Akt (Thr308) (D31E5E) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 488 Conjugate) #4412. |
