| 货号 | 9215L |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster/Pig/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC/F |
| 使用方法 | WB(1:1000) F (1:25) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). |
| 存放说明 | -20C |
| 计算分子量 | 43 |
Western blot analysis of extracts from Jurkat, C6, NIH/3T3 and COS cells, untreated or treated as indicated, using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (upper) or p38 MAPK Antibody #9212 (lower).western blot方法检测细胞提取物：未处理和按规定处理的Jurkat, C6, NIH/3T3、COS，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb (上图)和 p38 MAPK Antibody #9212 (下图)。 | |
Specificity of Phospho-Erk1/2, Phospho-p38 MAPK and Phospho-SAPK/JNK Rabbit mAb: Western blot analysis of extracts from NIH/3T3 cells treated with PDGF and UV, using Phospho-p38 MAPK Rabbit mAb #9215, Phospho-SAPK/JNK Rabbit mAb and Phospho-Erk1/2 Rabbit mAb.Phospho-Erk1/2, Phospho-p38 MAPK和Phospho-SAPK/JNK Rabbit mAb特异性分析western blot方法检测PDGF 、UV处理的NIH/3T3细胞提取物，使用的抗体是Phospho-p38 MAPK Rabbit mAb #9215, Phospho-SAPK/JNK Rabbit mAb和Phospho-Erk1/2 Rabbit mAb.。 | |
Flow cytometric analysis of Jurkat cells, untreated (blue) or anisomycin-treated (green), using Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mAb compared to a nonspecific negative control antibody (red).Flow cytometric方法检测细胞提取物：未经处理（蓝色）和茴香霉素处理的（绿色）Jurkat细胞，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit mA，红色曲线表示非特异性阴性对照抗体。 | |
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or anisomycin-treated (right), using Phospho-p38 MAPK (Thr180/Tyr182)(3D7) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).激光共聚焦荧光法检测HeLa细胞，左侧是未经处理的样品，右侧是经茴香霉素处理的样品，使用的抗体是Phospho-p38 MAPK (Thr180/Tyr182)(3D7) Rabbit mAb（呈绿色）。肌动蛋白丝被Alexa Fluor® 555 鬼笔环肽标记，呈红色。 |
