| 货号 | 9101L |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey/Mink/D.melanogaster/Zebrafish/Bovine/Pig/C.elegans |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC/F |
| 使用方法 | WB(1:1000) IP (1:50) F (1:200) IF-IC (1:250) |
| 供应商 | CST |
| 背景 | Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059. |
| 存放说明 | -20C |
| 计算分子量 | 42, 44 |
Specificity and sensitivity of Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody. The antibody reacts specifically with as little as 0.25 ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4 µg of nonphosphorylated p42 MAP kinase. | |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody兔多抗的特异性和灵敏度：该抗体对磷酸化的p42 MAPK的检测灵敏度可达0.25ng，对非磷酸化的p42 MAPK在多达4ug时也不会产生非特异结合。 | |
Phosphorylated MEK and Erk were assayed in human peripheral blood lymphocytes stimulated with PMA in the presence or absence of the Raf inhibitor BAY 37-9751 or the MEK inhibitor U0126 #9903. BAY 37-951 blocked PMA-stimulated phosphorylation of both MEK and Erk, consistent with inhibition at the level of Raf, while U0126 blocked phosphorylation of Erk only, consistent with inhibition at the level of MEK. From Chow, S. et al. (2001) Cytometry 46, 72-78. | |
在Raf抑制剂BAY 37-9751或MEK抑制剂U0126#9903存在的情况下，在经PMA刺激的人上皮血液淋巴细胞中用流式方法检测磷酸化的MEK、Erk。BAY 37-951阻断了PMA刺激的MEK、Erk两者的磷酸化，并与Raf水平抑制的结果相一致。而U0126只阻断Erk的磷酸化，并与MEK水平的抑制相一致。来自于Chow, S. 等 (2001) Cytometry 46, 72-78. | |
Flow cytometric analysis of Jurkat cells, untreated (green) or PMA-treated (blue), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody compared to a nonspecific negative control antibody (red). |
