| 货号 | 9701L |
| 反应种属 | Human/Mouse/Rat/Monkey/D.melanogaster/Zebrafish/S.cerevisiae |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | Phospho-Histone H3 (Ser10) Antibody detects endogenous levels of histone H3 only when phosphorylated at serine 10. The antibody does not cross-react with other phosphorylated histones or with acetylated histones. |
| 使用方法 | WB(1:1000) IHC-P (1:200) F (1:50) IF-IC (1:200) IHC-F (1:400) |
| 供应商 | CST |
| 背景 | Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11). |
| 存放说明 | -20C |
| 计算分子量 | 17 |
Immunohistochemical staining of phosphorylated histone H3 in paraffin-embedded human breast carcinoma showing nuclear localization using Phospho-Histone H3 (Ser10) Antibody. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析人源乳腺癌石蜡切片中histone H3的磷酸化。 | |
Immunocytochemical staining of NIH/3T3 cells, nocodazole-treated (2 ug/ml) and grown in 10% serum, showing cells undergoing mitosis using Phospho-Histone (Ser10) Antibody. 使用Phospho-Histone (Ser10) Antibody，免疫细胞化学染色NIH/3T3细胞，细胞经过nocodazole-treated (2 ug/ml)和10% serum培养。结果显示细胞正在经历有丝分裂。 | |
Western blot analysis of whole cell lysates of NIH/3T3 cells, untreated, treated with TSA (to induce histone acetylation), serum plus calyculin A (to induce phosphorylation of H3) or both, using Phospho-Histone H3 (Ser10) Antibody. 使用Phospho-Histone H3 (Ser10) Antibody，免疫印迹(Western blot)分析NIH/3T3全细胞裂解液，其分为未处理、处理了TSA (为了诱导组蛋白乙酰化)、处理serum plus calyculin A (为诱导H3的磷酸化)或两者都处理。 | |
Flow cytometric analysis of Phospho-Histone H3 (Ser10) Antibody staining of untreated (blue) or serum/calyculin treated (green) Ramos cells compared to a nonspecific negative control antibody (red). 与非特异阴性control antibody (红色)比较，使用Phospho-Histone H3 (Ser10) Antibody，流式细胞仪分析未处理的untreated (蓝色)或serum/calyculin treated (绿色) Ramos细胞。 | |
Flow cytometric analysis of untreated Jurkat cells, using Phospho-Histone H3 (Ser10) Antibody versus propidium iodide (DNA content). The box indicates phospho-histone H3 positive cells. 使用Phospho-Histone H3 (Ser10) Antibody和propidium iodide (DNA含量)，流式细胞仪分析未处理的Jurkat细胞。方框代表phospho-histone H3阳性细胞。 | |
Immunohistochemical analysis of paraffin-embedded human tonsil using Phospho-Histone H3 (Ser10) Antibody. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析人源扁桃体组织石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or lambda phosphatase-treated (right), using Phospho-Histone H3 (Ser10) Antibody. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析人源结肠癌组织石蜡切片，切片分为untreated (左图)或lambda phosphatase-treated (右图)。 | |
Immunohistochemical analysis of HT29 cells using Phospho-Histone H3 (Ser10) Antibody. Note the specific staining of mitotic cells. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析HT29细胞石蜡切片。说明有丝分裂细胞的特异性染色。 | |
Confocal microscopic image of a mitotic HeLa cell labeled with Phospho-Histone H3 (Ser10) Antibody (red) and Survivin (6E4) Mouse mAb #2802 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用Phospho-Histone H3 (Ser10) Antibody (红色)和Survivin (6E4) Mouse mAb #2802 (绿色)标记，共聚焦显微镜观察有丝分裂期HeLa细胞。蓝色= DRAQ5® #4084 (DNA荧光染料)。 | |
Immunohistochemical analysis of frozen H1650 xenograft, showing staining of mitotic cells using Phospho-Histone H3 (Ser10) Antibody. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析H1650 xenograft冰冻切片，结果显示有丝分裂期细胞的染色。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Histone H3 (Ser10) Antibody in the presence of control peptide (left) or Phospho-Histone H3 (Ser10) Blocking Peptide #1000 (right). 使用Phospho-Histone H3 (Ser10) Antibody，左图：control peptide或 右图：Phospho-Histone H3 (Ser10) Blocking Peptide #1000 ，免疫组化分析人源肺癌石蜡切片。 | |
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using Phospho-Histone H3 (Ser10) Antibody #9701. 使用Phospho-Histone H3 (Ser10) Antibody，免疫组化分析4T1 syngeneic mouse tumor石蜡切片。 |
